The CRI Acquires the Sony ID7000 Spectral Cell Analyzer

We are pleased to announce the installation of the Sony Biotechnology ID7000 Spectral Cell Analyzer at our center. This next-generation cytometer represents a major advancement in our cell analysis capabilities, offering high-performance spectral flow cytometry tailored to the most demanding biomedical research applications.

Unlike conventional systems, the ID7000 is based on full-spectrum analysis: instead of isolating specific fluorescence bands using filters, it captures the entire emission spectrum of each fluorochrome. This approach enables the distinction of fluorochromes with highly similar spectral profiles, paving the way for panels of over 44 colors.

The system is configured with six lasers: 320 nm, 355 nm, 405 nm, 488 nm, 561 nm, and 633 nm. This high-resolution configuration allows for the precise identification of cell subpopulations and the detection of rare or low-expression markers. The 320 nm laser specifically improves panel resolution by enhancing the spectral unmixing process.

The ID7000’s integrated software is designed to streamline workflows, from daily calibration to data analysis. Key features include:

  • Automated setup and quality control at the beginning of each day;

  • Automated acquisition mode (24-tube rack, 96-well plates [U, V, flat-bottom], 384-well plates);

  • Standardization mode (enabling multi-site studies with identical configurations);

  • A spectral reference library to avoid repeated single-color control acquisitions;

  • Advanced autofluorescence correction and unmixing tools for more reliable data;

  • An integrated AutoSampler with clog detection for walkaway acquisition.

A New Asset for Our Research Projects

With its advanced performance, the ID7000 enables us to pursue innovative experimental approaches across a range of research fields, including:

  • Deep immunophenotyping

  • Biomarker detection in rare cell populations

  • Study of immune responses and cytokine expression

  • Analysis of extracellular vesicles or viral particles (resolution down to 160 nm)

Combining high sensitivity, operational flexibility, and reliability, this new equipment significantly enhances the technological capabilities of our laboratory.

The ID7000 is available in Room 414, 4th floor.

To learn more about the ID7000 cytometer:
➡️ Full technical data sheet on the Sony Biotechnology website
📄 Download the PDF brochure