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Photo equipe rautou 2023

Vessels in liver diseases (Atip-Avenir)

Team Rautou

Department Hepato-Gastroenterology Department

 

Leader(s)

 

Line-up

 

Presentation

Our team is working on the role of vessels in liver disease. We are interested in rare vascular liver diseases (Budd-Chiari syndrome, extrahepatic portal vein thrombosis and porto-sinusoidal vascular disorder), metabolic dysfunction-associated steatohepatitis and cirrhosis.

Current research directions :
– Liver sinusoidal endothelial cells in the progression of liver diseases
– Extracellular vesicles in liver diseases
– Porto-sinusoidal vascular disease

 

Liver sinusoidal endothelial cells in the progression of liver diseases

Recent advances indicate that intestinal and visceral adipose tissues play a key role in liver diseases. This suggests that substances are released by these tissues, influencing liver function. Liver endothelial cells are among the first cells to encounter blood from intestinal and visceral fat tissues. They are an active interface between blood and other hepatic cells. However, the role of liver endothelial cells in liver diseases is paradoxically very poorly understood.

We recently demonstrated that autophagy is defective in liver endothelial cells of patients with Metabolic dysfunction-associated steatohepatitis (MASH) and that this defect is induced by inflammatory mediators present in the portal blood of patients with metabolic syndrome
We also demonstrated that deficiency in autophagy induces liver endothelial cell alterations responsible for liver inflammation, liver cell death and liver fibrosis, thus promoting NASH development (Hammoutene A. J Hepatol. 2019).

Left :Primary murine liver sinusoidal endothelial cells take up acLDL with high efficiency

Right : Liver sinusoidal endothelial cells in murine liver section (green)

Extracellular vesicles in liver diseases

Extracellular vesicles are membrane-bound vesicles released by cells into the extracellular space. They include apoptotic bodies, microvesicles and exosomes : called now large and small vesicles. Extracellular vesicles are able to transmit information from one cell to another. We are currently determining the role of extracellular vesicles in progression and complications of liver diseases. To do so, we analyze their roles in intercellular communication between the liver and other organs, as well as among the liver itself. 

We have recently demonstrated that microvesicles from red blood cells carrying JAK2V671F mutation transfer a protein (myeloperoxidase) to endothelial cells thus increasing arterial response. This data can explain the increase of cardiovascular events in patients with JAK2V617F myeloproliferative neoplasms (Poisson J. J Clin Invest. 2020).

Electronic microscopy view from extracellular vesicles in liver vessels

Extracellular vesicles can also be used as biomarkers. We demonstrated that hepatocyte microvesicles can predict the course of patients with cirrhosis (Bissonnette J. Hepatology. 2017 & Payancé A. Hepatology 2018 & Thietart S. Journal of hepatology. 2020).

In 2023, we have shown that hepatocyte-derived extracellular vesicles are also able to predict liver-related events at 2 years in patients with Child-Pugh class A alcohol-related cirrhosis (Elkrief L. J Hepatol 2023)

Using proteomics on extracellular vesicles, we have identified several new biomarkers that can be used to define sub-populations of extracellular vesicles that could improve the diagnosis of liver diseases.

We are now validating these results in large cohorts of patients: DECISION cohort; PROMICE; RHU QUID NASH.

 

Porto-sinusoidal vascular disorder

Previously called non-cirrhotic portal hypertension or nodular regenerative hyperplasia, is a rare disease characterized by alterations in the portal veins and/or sinusoids leading in some patients to portal hypertension and its complications. We are studying the pathophysiology of this disease as well as the future of patients with this disease. Pr. Pierre-Emmanuel Rautou is responsible of unity of Catheterisme hepatique in Beaujon Hospital.

 

Podcast of Prof PE Rautou on porto-sinusoidal vascular disease: link

 

 

 

Landmark publications

  • Publication date : 09 June 2023 More

    Hepatocyte-derived biomarkers predict liver-related events at 2 years in Child-Pugh class A alcohol-related cirrhosis
    J Hepatol

    Authors : Elkrief Laure Nathalie Ganne-Carrié Manceau Hana Tanguy Marion Shantha Ram Valainathan Alix Riescher-Tuczkiewicz Biquard Louise Nathalie Barget Cendrine Chaffaut Alexandre Louvet Paradis Valérie Marianne Ziol Rikke Bæk Malene Møller Jørgensen Guillaume Van Niel Pierre-Michael Coly Hammoutene Adel Fanny Dujardin Katell Peoc'h Thierry Poynard Sylvie Chevret Rautou Pierre-Emmanuel CIRRAL group

  • Publication date : 01 December 2020 More

    Extracellular vesicles as biomarkers in liver diseases: A clinician’s point of view
    J Hepatol.

    Authors : Thietart Sara Rautou Pierre-Emmanuel

  • Publication date : 01 March 2020 More

    A defect in endothelial autophagy occurs in patients with non-alcoholic steatohepatitis and promotes inflammation and fibrosis
    J Hepatol.

    Authors : Hammoutene Adel Biquard Louise Juliette Lasselin Marouane Kheloufi Tanguy Marion Anne-Clémence Vion Jules Mérian Nathalie Colnot Xavier Loyer Alain Tedgui Patrice Codogno Lotersztajn Sophie Paradis Valérie Chantal M Boulanger Rautou Pierre-Emmanuel

  • Publication date : 01 June 2019 More

    Role of liver sinusoidal endothelial cells in non-alcoholic fatty liver disease.
    J Hepatol.

    Authors : Hammoutene Adel Rautou Pierre-Emmanuel

  • Publication date : 01 October 2018 More

    Hepatocyte microvesicle levels improve prediction of mortality in patients with cirrhosis.
    Hepatology

    Authors : Payancé A Silva-Junior G Bissonnette J Tanguy Marion Pasquet B Levi C Roux O Nekachtali O Baiges A Hernández-Gea V Laouénan C Lebrec D ALBUQUERQUE Miguel Paradis Valérie Moreau Richard Valla Dominique Durand François Boulanger CM Garcia-Pagan JC Rautou Pierre-Emmanuel

  • Publication date : 01 August 2017 More

    A prospective study of the utility of plasma biomarkers to diagnose alcoholic hepatitis.
    Hepatology.

    Authors : Bissonnette J Altamirano J Devue C Roux O Payancé A Lebrec D Bedossa Pierre Valla Dominique Durand François Ait-Oufella H Sancho-Bru P Caballeria J Ginès P Boulanger CM Bataller R Rautou Pierre-Emmanuel

  • Publication date : 04 January 2017 More

    Liver sinusoidal endothelial cells: physiology and role in liver diseases
    J Hepatol.

    Authors : Poisson Johanne Lemoinne S Boulanger C Durand François Moreau Richard Valla Dominique Rautou Pierre-Emmanuel

  • Publication date : 15 January 2016 More

    Hepatocyte Tissue Factor Contributes to the Hypercoagulable State in a Mouse Model of Chronic Liver Injury.
    J Hepatol.

    Authors : Rautou Pierre-Emmanuel Tatsumi K Antoniak S Owens AP Sparkenbaugh E Holle LA Wolberg AS Kopec AK Pawlinski R Luyendyk JP Mackman N Poisson Johanne Lemoinne S Boulanger C Durand François Moreau Richard Valla Dominique Rautou Pierre-Emmanuel

  • Publication date : 14 June 2014 More

    The emerging roles of microvesicles in liver diseases.
    Nat Rev Gastro Hepat

    Authors : Lemoinne S Thabut D Housset C Moreau Richard Valla Dominique Boulanger CM Rautou Pierre-Emmanuel

  • Publication date : 30 March 2012 More

    Abnormal Plasma Microparticles Impair Vasoconstrictor Responses in Patients with Cirrhosis.
    Gastroenterology.

    Authors : Rautou Pierre-Emmanuel Bresson J Sainte-Marie Y Vion AC Paradis Valérie Renard JM Devue C Heymes C Letteron Philippe Elkrief Laure Lebrec D Valla Dominique Tedgui A Moreau Richard Boulanger CM

 
 

Site de référence pour les Appels à Projets


Les dernières publications marquantes

  • Publication date : 01 May 2024 More

    Food additive emulsifiers and the risk of type 2 diabetes: analysis of data from the NutriNet-Santé prospective cohort study
    Lancet Diabetes Endocrinol.

    Authors : Clara Salame Guillaume Javaux Laury Sellem Viennois Emilie Fabien Szabo de Edelenyi Cédric Agaësse Alexandre De Sa Inge Huybrechts Fabrice Pierre Xavier Coumoul Chantal Julia Emmanuelle Kesse-Guyot Benjamin Allès Léopold K Fezeu Serge Hercberg Mélanie Deschasaux-Tanguy Emmanuel Cosson Sopio Tatulashvili Benoit Chassaing Bernard Srour Mathilde Touvier

  • Publication date : 09 April 2024 More

    Prognosis algorithms for acute decompensation of cirrhosis and ACLF
    Liver Int.

    Authors : Valainathan Shantha Qing Xie Vicente Arroyo Rautou Pierre-Emmanuel

  • Publication date : 01 March 2024 More

    Hepatocellular carcinoma in patients with non-alcoholic fatty liver disease is associated with heterogeneous pattern of fat infiltration in skeletal muscles
    Eur Radiol.

    Authors : Maxime Nachit Dioguardi Burgio Marco Abyzov Anton Garteiser Philippe Paradis Valérie Vilgrain Valérie Isabelle Leclercq Van Beers Bernard