The CRI Flow Cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers training in sample acquisition, processing and analysis techniques.
It provides self-service access to four analyzers and a sorter, as well as occasional assistance with projects requiring mastery of this technology.
Image cytometry is only available as a service (data acquisition by an engineer).
The Charter must be completed, signed and submitted to the platform managers for validation before any use.
Services on offer
– Analysis
- Multiple membrane and intracellular markings
- Signaling pathways
- Cell cycle
- Apoptosis
- Multiplex molecular analysis
- Analysis of transfected cells .
– Image cytometry (Imagestream MKII)
– Training
– Help in setting up or improving an experimental protocol
– Help in analyzing and interpreting results
Our equipment
Spectral cytometer
– Sony ID7000 6 lasers
320 nm, 355 nm, 405 nm, 488 nm, 561 nm and 633 nm
Flow cytometer :
– BD Fortessa X20: 22 parameters (5 lasers)
Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm), UV (365nm)
– BD Fortessa: 18 parameters (4 lasers)
Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm)
– Guava Easycyte6 parameters (2 lasers)
Blue (488 nm), Red (633 nm)
– Imagestream MKII ( 5 lasers)
Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (642 nm), UV (375nm)
X20, X40, X60
Sorter :
– Melody ( 3 lasers)
Blue (488 nm), Green-yellow (561 nm), Red (633 nm)
Tri 2 channels, cloning module
Access on request
– BD Aria III sorter: 18 parameters (4 lasers)
Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (633 nm) on request
Coming to the platform
Inflammation Research Center
site Bichat – 4th floor, rooms 456 and 414
