The CRI flow cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers the possibility of training in the techniques of acquisition, processing and analysis of samples.

It provides self-service, four analyzers and a sorter but also offers ad hoc assistance in projects requiring mastery of this technology.

Image cytometry is only offered as part of a service (data acquisition by an engineer)

The Charter must be completed, signed and submitted to the platform managers for validation before any use.

Services offered

Analyses

  1. Multi membrane and intracellular markings
  2. Signaling lanes
  3. Cell cycle
  4. Apoptosis
  5. Molecular analysis in multiplex
  6. Analysis of transfected cells ..

Image cytometry (Imagestream MKII)

Training

Help in setting up or improving an experimental protocol

Help in the analysis and interpretation of results

 

Our equipment

– BD Fortessa X20: 22 parameters (5 lasers)
Violet (405nm), Blue (488nm), Green-yellow (561nm), Red (638nm), UV (365nm)

BD Fortessa: 18 parameters (4 lasers)
Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm)

– BD Canto II: 10 parameters (3 lasers)
Violet (405 nm), Blue (488 nm), Red (633 nm)

– Guava Easycyte : 6 parameters (2 lasers)
Blue (488 nm), Red (633 nm)

– Melody (3 lasers)
Blue (488 nm) , Green-yellow (561 nm) , Red (633 nm)
2-way sorting, cloning module

– Imagestream MKII ( (5 lasers)
Violet (405nm), Blue (488nm), Green-yellow (561nm), Red (642nm), UV (375nm)
X20, X40, X60

Access possible on request

– BD Aria III Sorter: 18 parameters (4 lasers)
Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (633 nm) on request

Come to the platform

Center for Research on Inflammation
Bichat site – 4th floor, room 456

 

Reserve

 

 

Scientific manager of the platform


Technical manager


Engineer