In this drop-down list, you will find the various equipment that make up the IMA’CRI platform:
Confocal Zeiss LSM 780 Airyscan
The Zeiss LSM 780 inverted confocal microscope, controlled by ZEN software and equipped with a thermostatic chamber, is fully motorized and enables sub-cellular observation of fixed samples or living cells. The microscope is equipped with a spectral strip for simultaneous observation of up to 8 colors, and a high-resolution detector to improve resolution by a factor of 1.7.
Related technologies :
2D imaging
3D imaging
FRAP photoactivation
Ratiometric FRET
Spectral imaging
High-resolution imaging
Available lenses
| Lenses | Digital opening | Immersion |
| X10 Plan Apo | 0.45 DF= 2.1 mm | sec |
| X40 W C-Apo | 1.2 DF= 280 µm | water |
| X40 DIC Plan Apo | 1.3 DF= 200 µm | oil |
| X63 DIC Plan Apo | 1.4 DF= 190 µm | oil |
Laser sources
– 405nm diode, 30mW
– Argon laser, lines at 458, 488, 514 nm, 25mW
– 561nm diode, 20 mW
– Diode 633 nm, 5mW
Detectors
2 PMTs (multi-alkali coated photocathodes), one of which is cooled
1 PMT transmission
1 cooled 32-channel multiPMT (GaAsP photocathode) for spectral detection.
1 Airyscan detector comprising 32 GaAsP honeycomb detectors
Confocal Multiphoton Leica SP8
The Leica Sp8 upright confocal microscope, controlled by LAS X software, enables sub-cellular observation of fixed samples or living cells. It is equipped with deconvolution software for enhanced resolution.
Related technologies:
2D imaging
3D imaging
Multiphoton imaging
Available lenses
| Lenses | Digital opening | Immersion |
| X25 PL IRAPO | 0.95 DT= 2.5 mm | water |
| X40 PLAN APO | 1.3 DT=240 µm | oil |
| X63 PLAN APO | 1.4 DT = 280 µm | oil |
Laser sources
– 405 nm diode, 50 mW
– Argon Multilines at 458, 488, 514 nm, 65 mW
– Diode at 561 nm, 20 mW
– Diode 633 nm, 10 mW
– Chameleon Vision II pre-compensated IR Coherent laser tunable from 680 to 1080 nm. It features (500-550, 565-605) for maximum observation.
Detectors
It is equipped with 2 PMTs and a HyD
Videomicroscope-TIRF Zeiss Axiovert 200
This inverted microscope can perform transmitted light and fluorescence times lapses on living cells. It can also be used for TIRF.
Filters
GFP/mRFP EX DBP 480/30 + 565/25. EM DBP 525/31 + 616/57
It is equipped with :
– a thermostatically-controlled, CO2-regulated chamber for studies on living cells
– an XYZ motorized stage
– high-resolution Cool Snap HQ2 camera
– an Evolve EMCCD camera
– a DG4 with Fura2 filter for Calcium detection
Available lenses
| Lenses | Digital opening | Immersion |
| X2.5 Plan-Neofluar | 0.075 df=9.5 mm | sec |
| X5 Plan-Neofluar | 0.16 df= 18.5 mm | sec |
| X10 Ph 1 Plan-Neofluar | 0.5 df= 5.2 mm | sec |
| X20 Ph2 Plan-Neofluar | 0.5 df= 2 mm | sec |
| X40 DIC Plan-Apo | 1.3 df= 0.2 mm | oil |
| X63DIC Plan-Apo | 1.4 df= 0,19 mm | oil |
| X100 DIC Plan-Apo | 1.46 df= 0,10 mm | oil |
Filter blocks
| Singleband HE | Multiband | Multiband |
| DAPI EM EX G 365BP 445/50 | GFP/mRFP | DAPI/GFP/Cy3/Cy5 |
| eGFP EX BP 470/40, EM BP 525/50 | CFP/YFP | |
| CFP EX BP 550/25, EM BP 480/40 | Fura 2, EX BP 340/30, EM BP 690/50 | |
| YFP EX BP 500/25, EM BP 535/30 | ||
| Cya3 EX BP 436/25, EM BP 605/70 | ||
| Cya5 EX BP 640/30, EM BP 690/50 |
Image analysis software
Imaris is a 3D/4D reconstruction software package published by Bitplane. It can be used to visualize images taken using photonic microscopy, by volume and surface reconstruction. It is freely available on the Plate-Forme after reservation on the CRI resources site.
Fiji is very comprehensive for image analysis (co-location, stack processing, colorimetry, edge search, segmentation, z projection, overlay, filters, 3D, .AVI .GIF animation creation, 3D deconvolution, …) and allows you to create macros.
Official website: https://imagej.net/Fiji