Authors
The thesis work of Elia Gigante and iMAP platform engineer Hélène Cazier from Valérie Paradis ‘ team has enabled the molecular characterization of hepatocholangiocarcinomas, a subtype of malignant primary liver tumors that are difficult to diagnose due to their significant intratumoral heterogeneity. Comparison of molecular profiles acquired by MALDI imaging on all fixed tissue sections identified the presence of both tumor components (hepatocellular carcinoma and cholangiocarcinoma), and revealed intricate microscopic tumor areas not spotted under the microscope. This work presents a new application of MALDI imaging ( in silico imaging) offering a virtual multiplex immunohistochemistry approach.
Here is the type of image and analysis obtained with MALDI imaging:
Segmentation analysis superimposed on immunohistochemistry performed on successive slices of a hepatocholangiocarcinoma. (A) Pathological annotations indicating areas of cholangiocarcinoma (green), hepatocellular carcinoma (red), fibrosis in cholangiocarcinoma (blue) * MVI. (B) Segmentation analysis from MALDI imaging identifying 4 distinct. areas of cholangiocarcinoma-related fibrosis (blue); HCC (red); iCCA (green); and fibrosis around cirrhotic nodules (yellow).