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Leader(s)
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Dang Pham My-Chan
- 01 57 27 74 72
- my-chan.dang@inserm.fr
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Hurtado-Nedelec Maria Margarita
- 01 40 25 70 26
- maria.hurtado-nedelec@aphp.fr
Phagocytes, NOX and ROS in inflammation (PNRI)
Team Dang / Hurtado Nedelec
Scientific axes : Immunology, Signaling and Cellular Dynamics
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Researcher(s)
Research assistants
Post-doctoral fellows
Presentation
Our team is interested in studying molecular and cellular mechanisms of inflammation, focusing on the regulationof the main reactive oxygen species (ROS) producing enzymes, the NADPH oxidases (NOXs) and their role in thephysiopathology of inflammatory diseases such as rheumatoid arthritis (RA) and inflammatory bowel diseases (IBD). When their production is controlled, reactive oxygen species (ROS) are involved in many important physiological functions. Thus ROS produced by the phagocyte NADPH oxidase, NOX2, in neutrophils, monocytes and macrophages are required for their bactericidal function, and importantly in dendritic cells, NOX2 has a critical role in antigen cross-presentation. Homologues of NOX2, such as NOX1, are also expressed in epithelial cells, but their physiological role is not fully understood. On the other hand, it is clear that excessive or inappropriate ROS production can induce severe tissue injury that participates to the physiopathology of inflammatory diseases. Deciphering mechanisms underlying activation of NOX2 in phagocytes and NOX1 in epithelial cells is essential to identify novel therapeutic targets in inflammation. The projects of the team aim to understand the regulation of NOX2 in neutrophils, monocytes/macrophages and dendritic cells and NOX1 in epithelial cells by pro- and anti-inflammatory agents; and to translate our basic research to animal models of inflammation, human phagocytic disorders and human inflammatory diseases. Our project combines basic and clinical research and involves several collaborations with teams in the Center for Research on Inflammation (CRI) and Bichat hospital divisions.
Principal research axis:
Our work is conducted following three main axis : 1)-To understand the regulation of the NADPH oxidases NOX in neutrophils, monocytes, macrophages, dendritic cells and epithelial cells in response to pro- and anti-inflammatory agents. 2)-To study polymorphism of NOX and regulators and identify new genetic factors involved in systemic inflammation. 3-To study the role of the NOX2, NOX1 and newly identified proteins in inflammatory process: by investigating their activation in phagocytic disorders, human inflammatory diseases and in animal models of inflammation.
Landmark publications
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Publication date : 19 September 2023 More
TNFα counteracts interleukin-10 anti-inflammatory pathway through the NOX2-Lyn-SHP-1 axis in human monocytes
Redox Biol.
Authors : Ben-Khemis Marwah Liu Dan Pintard Coralie Song Zhuoyao Hurtado-Nedelec Maria Margarita Marie Jean-Claude El Benna Jamel Dang Pham My-Chan
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Publication date : 31 May 2023 More
CARD9 in neutrophils protects from colitis and controls mitochondrial metabolism and cell survival
Gut.
Authors : Camille Danne Chloé Michaudel Jurate Skerniskyte Julien Planchais Aurélie Magniez Allison Agus Marie-Laure Michel Bruno Lamas Gregory Da Costa Madeleine Spatz Cyriane Oeuvray Chloé Galbert Maxime Poirier Yazhou Wang Alexia Lapière Nathalie Rolhion Tatiana Ledent Cédric Pionneau Solenne Chardonnet Floriant Bellvert Edern Cahoreau Amandine Rocher Rafael Rose Arguello Carole Peyssonnaux Sabine Louis Mathias L Richard Philippe Langella El Benna Jamel Benoit Marteyn Harry Sokol
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Publication date : 16 January 2023 More
ROCK2 interacts with p22phox to phosphorylate p47phox and to control NADPH oxidase activation in human monocytes
PNAS
Authors : Asma Tlili Pintard Coralie Hurtado-Nedelec Maria Margarita Liu Dan Viviana Marzaioli Thieblemont Nathalie Dang Pham My-Chan El Benna Jamel
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Publication date : 01 January 2022 More
Impaired p47phox phosphorylation in neutrophils from p67phox-deficient chronic granulomatous disease patients
Blood.
Authors : Sahra Amel Belambri Viviana Marzailoli Hurtado-Nedelec Maria Margarita Pintard Coralie Shiyu Liang Yezhou Liu Tarek Boussetta Gougerot-Pocidalo Marie-Anne Richard D Ye Dang Pham My-Chan El Benna Jamel
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Publication date : 28 January 2022 More
Metformin Inhibits ROS Production by Human M2 Macrophages via the Activation of AMPK
Biomedicines
Authors : Rana M Nassif Elias Chalhoub Pia Chedid Hurtado-Nedelec Maria Margarita Elia Raya Dang Pham My-Chan Marie Jean-Claude El Benna Jamel
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Publication date : 10 January 2022 More
Protein Kinase CK2 acts as a molecular brake to control NADPH oxidase 1 activation and colon inflammation
Cell Mol Gastroenterol Hepatol.
Authors : Liu Dan Marie Jean-Claude Pelletier Anne-Laure Song Zhuoyao Ben-Khemis Marwah Kaouthar Boudiaf Pintard Coralie Thibaut Leger Samuel Terrier Guillaume Chevreux El Benna Jamel Dang Pham My-Chan
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Publication date : 05 September 2021 More
Impaired respiratory burst contributes to infections in PKCδ-deficient patients
J Exp Med.
Authors : Anna-Lena Neehus Kunihiko Moriya Alejandro Nieto-Patlán Tom Le Voyer Romain Lévy Ahmet Özen Elif Karakoc-Aydiner Safa Baris Alisan Yildiran Engin Altundag Manon Roynard Kathrin Haake Mélanie Migaud Karim Dorgham Guy Gorochov Laurent Abel Nico Lachmann Figen Dogu Sule Haskologlu Erdal İnce El Benna Jamel Gulbu Uzel Ayca Kiykim Kaan Boztug Marion R Roderick Mohammad Shahrooei Paul A Brogan Hassan Abolhassani Gonca Hancioglu Nima Parvaneh Alexandre Belot Aydan Ikinciogullari Jean-Laurent Casanova Anne Puel Jacinta Bustamante
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Publication date : 10 August 2021 More
Uncoupling of IL-6 signaling and LC3-associated phagocytosis drives immunoparalysis during sepsis
Cell Host Microbe.
Authors : Tonia Akoumianaki Katerina Vaporidi Eleni Diamantaki Frédéric Pène Remi Beau Mark S Gresnigt Marina Gkountzinopulou Maria Venichaki Elias Drakos El Benna Jamel George Samonis Kieu T T Le Vinod Kumar Dimitrios Georgopoulos Frank L van de Veerdonk Mihai G Netea Jean-Paul Latge Georgios Chamilos
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Publication date : 02 August 2020 More
The protein kinase A negatively regulates reactive oxygen species production by phosphorylating gp91phox/NOX2 in human neutrophils
Free Radic Biol Med
Authors : Houssam Raad Hussein Mouawia Hamad Hassan Mohamed El-Seblani Riad Arabi-Derkawi Tarek Boussetta Gougerot-Pocidalo Marie-Anne Dang Pham My-Chan El Benna Jamel
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Publication date : 30 June 2020 More
Apocynin prevents GM-CSF-induced-ERK1/2 activation and -neutrophil survival independently of its inhibitory effect on the phagocyte NADPH oxidase NOX2
Biochem Pharmacol.
Authors : Pintard Coralie Ben-Khemis Marwah Liu Dan Dang Pham My-Chan Hurtado-Nedelec Maria Margarita El Benna Jamel
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Publication date : 29 February 2020 More
Mast cell chymase protects against acute ischemic kidney injury by limiting neutrophil hyperactivation and recruitment
Kidney Int.
Authors : Lydia Celia Madjene Luca Danelli Albert Dahdah Vibhushan Shamila Bex-Coudrat Julie Lebourdais-Pacreau Emeline Celine Vaugier Julien Claver Loïc Rolas Maguelonne Pons Iris Karina Madera-Salcedo Walid Beghdadi El Ghoneimi Alaa Benhamou Marc Launay Pierre Magnus Abrink Gunnar Pejler Ivan Cruz Moura Charles Nicolas Daugas Eric Perianin Axel Blank Ulrich
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Publication date : 21 January 2020 More
The dual role of ROS-generating NADPH oxidases in gastrointestinal inflammation and therapeutic perspectives.
Antioxidants & Redox Signaling.
Authors : Dang Pham My-Chan Rolas L El Benna Jamel
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Publication date : 31 October 2019 More
The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils.
Frontiers In Immunology.
Authors : Liu Min Bedouhene Samia Hurtado-Nedelec Maria Margarita Pintard Coralie Dang Pham My-Chan Yu S El Benna Jamel
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Publication date : 05 September 2019 More
Cytosolic PCNA interacts with p47phox and controls NADPH oxidase NOX2 activation in neutrophils.
J Exp Med.
Authors : Ohayon D De Chiara A Dang Pham My-Chan Thieblemont N Chatfield S Marzaioli V Burgener SS Mocek J Candalh C Pintard Coralie Tacnet-Delorme P Renault G Lagoutte I Favier M Walker Francine Hurtado-Nedelec Maria Margarita Desplancq D Weiss Emmanuel Benarafa C Housset D Marie Jean-Claude Frachet P El Benna Jamel Witko-Sarsat V
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Publication date : 28 February 2019 More
The Kinesin Light Chain-Related Protein PAT1 Promotes Superoxide Anion Production in Human Phagocytes.
J Immunol.
Authors : Arabi-Derkawi R O'Dowd Y Cheng N Rolas L Boussetta T Raad H Marzaioli V Pintard Coralie Fasseu M Kroviarski Y Belambri SA Dang Pham My-Chan Ye RD Gougerot-Pocidalo Marie-Anne El Benna Jamel
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Publication date : 01 September 2018 More
NOX1-derived ROS drive the expression of Lipocalin-2 in colonic epithelial cells in inflammatory conditions
Mucosal Immunology.
Authors : Makhezer N Ben-Khemis Marwah Liu Dan Khichane Y Marzaioli V Tlili A Mojallali Marjan Pintard Coralie Letteron Philippe Hurtado-Nedelec Maria Margarita El Benna Jamel Marie Jean-Claude Sannier A Pelletier Anne-Laure Dang Pham My-Chan