{"id":65324,"date":"2023-03-09T13:04:58","date_gmt":"2023-03-09T12:04:58","guid":{"rendered":"https:\/\/cri1149.fr\/?post_type=publications&#038;p=65324"},"modified":"2025-09-11T16:47:57","modified_gmt":"2025-09-11T14:47:57","slug":"ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development","status":"publish","type":"publication","link":"https:\/\/cri1149.fr\/en\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/","title":{"rendered":"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development"},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false},"category_publication":[],"class_list":["post-65324","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"Front Immunol.","date_de_publication":"20220629","numero_doi":"","equipe":[101],"auteurs-liste":[{"texte_libre":false,"auteur-lien":13209,"auteur-text":""},{"texte_libre":false,"auteur-lien":14854,"auteur-text":""},{"texte_libre":false,"auteur-lien":16167,"auteur-text":""},{"texte_libre":false,"auteur-lien":14855,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Hajime Karasuyama"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Kensuke Miyake"},{"texte_libre":false,"auteur-lien":13037,"auteur-text":""},{"texte_libre":false,"auteur-lien":13030,"auteur-text":""},{"texte_libre":false,"auteur-lien":13058,"auteur-text":""},{"texte_libre":false,"auteur-lien":13051,"auteur-text":""}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/pubmed.ncbi.nlm.nih.gov\/35844602\/"}],"paragraphe":"[:fr]<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\">Tissue-specific mouse models are essential tools to decipher the role of each cell compartment and\/or their expressed genes in the pathophysiology of diseases. Here, we describe a new knock-in mouse model allowing expression of both the fluorescent protein tdTomato and the CRE recombinase selectively in the basophil compartment under the control of the Mcpt8 gene. These \"CT-M8\" mice did not show any abnormalities in their peripheral distribution of major immune cell populations nor their basophil function. CT-M8 mice allowed the identification of basophils by immunofluorescence and flow cytometry and basophil-specific Cre-mediated floxed gene deletion. Breeding of our CT-M8 mice with the\u00a0<i>ROSA26<sup>flox-stop-DTA<\/sup><\/i>\u00a0mice led to the generation of basophil-deficient mice with no detectable abnormalities in other cell compartments. These mice were then used to document basophil involvement in systemic lupus erythematosus (SLE) pathophysiology since we previously reported by transient depletion of these cells during the course of an ongoing disease that they support and amplify autoantibody production in two distinct lupus-like mouse models (<i>Lyn<sup>-\/-<\/sup><\/i>\u00a0and pristane-induced). Here, constitutive basophil deficiency prevented pristane-induced lupus-like disease development by limiting autoantibody titers and renal damages. Therefore, basophils have a nonredundant role in pristane-induced lupus-like disease and are involved in both its induction and amplification. This CT-M8 new mouse model will allow us to finely decipher the role of basophils and their expressed genes in health and disease.<\/p>\r\n<\/div>[:]","paragraphe_en":"[:fr]<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\">Tissue-specific mouse models are essential tools to decipher the role of each cell compartment and\/or their expressed genes in the pathophysiology of diseases. Here, we describe a new knock-in mouse model allowing expression of both the fluorescent protein tdTomato and the CRE recombinase selectively in the basophil compartment under the control of the Mcpt8 gene. These \"CT-M8\" mice did not show any abnormalities in their peripheral distribution of major immune cell populations nor their basophil function. CT-M8 mice allowed the identification of basophils by immunofluorescence and flow cytometry and basophil-specific Cre-mediated floxed gene deletion. Breeding of our CT-M8 mice with the\u00a0<i>ROSA26<sup>flox-stop-DTA<\/sup><\/i>\u00a0mice led to the generation of basophil-deficient mice with no detectable abnormalities in other cell compartments. These mice were then used to document basophil involvement in systemic lupus erythematosus (SLE) pathophysiology since we previously reported by transient depletion of these cells during the course of an ongoing disease that they support and amplify autoantibody production in two distinct lupus-like mouse models (<i>Lyn<sup>-\/-<\/sup><\/i>\u00a0and pristane-induced). Here, constitutive basophil deficiency prevented pristane-induced lupus-like disease development by limiting autoantibody titers and renal damages. Therefore, basophils have a nonredundant role in pristane-induced lupus-like disease and are involved in both its induction and amplification. This CT-M8 new mouse model will allow us to finely decipher the role of basophils and their expressed genes in health and disease.<\/p>\r\n<\/div>[:]","documents":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development - CRI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development - CRI\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-11T14:47:57+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\\\/\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\\\/\",\"name\":\"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development - CRI\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#website\"},\"datePublished\":\"2023-03-09T12:04:58+00:00\",\"dateModified\":\"2025-09-11T14:47:57+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/cri1149.fr\\\/publication\\\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/cri1149.fr\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Publications\",\"item\":\"https:\\\/\\\/cri1149.fr\\\/publications\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#website\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/\",\"name\":\"CRI - Centre de Recherche sur l'Inflammation\",\"description\":\"Centre de Recherche sur l&#039;Inflammation\",\"publisher\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/cri1149.fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#organization\",\"name\":\"CRI1149\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/wp-content\\\/uploads\\\/2014\\\/11\\\/8.4.2014-logo-CRI.jpg\",\"contentUrl\":\"https:\\\/\\\/cri1149.fr\\\/wp-content\\\/uploads\\\/2014\\\/11\\\/8.4.2014-logo-CRI.jpg\",\"width\":215,\"height\":176,\"caption\":\"CRI1149\"},\"image\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\",\"https:\\\/\\\/www.linkedin.com\\\/company\\\/cri-centre-de-recherche-sur-l-inflammation\\\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development - CRI","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/","og_locale":"en_US","og_type":"article","og_title":"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development - CRI","og_url":"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/","og_site_name":"CRI","article_publisher":"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","article_modified_time":"2025-09-11T14:47:57+00:00","twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/","url":"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/","name":"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development - CRI","isPartOf":{"@id":"https:\/\/cri1149.fr\/#website"},"datePublished":"2023-03-09T12:04:58+00:00","dateModified":"2025-09-11T14:47:57+00:00","breadcrumb":{"@id":"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/cri1149.fr\/publication\/ct-m8-mice-a-new-mouse-model-demonstrates-that-basophils-have-a-nonredundant-role-in-lupus-like-disease-development\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/cri1149.fr\/en\/"},{"@type":"ListItem","position":2,"name":"Publications","item":"https:\/\/cri1149.fr\/publications\/"},{"@type":"ListItem","position":3,"name":"CT-M8 Mice: A New Mouse Model Demonstrates That Basophils Have a Nonredundant Role in Lupus-Like Disease Development"}]},{"@type":"WebSite","@id":"https:\/\/cri1149.fr\/#website","url":"https:\/\/cri1149.fr\/","name":"CRI - Centre de Recherche sur l'Inflammation","description":"Centre de Recherche sur l&#039;Inflammation","publisher":{"@id":"https:\/\/cri1149.fr\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/cri1149.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/cri1149.fr\/#organization","name":"CRI1149","url":"https:\/\/cri1149.fr\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cri1149.fr\/#\/schema\/logo\/image\/","url":"https:\/\/cri1149.fr\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","contentUrl":"https:\/\/cri1149.fr\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","width":215,"height":176,"caption":"CRI1149"},"image":{"@id":"https:\/\/cri1149.fr\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","https:\/\/www.linkedin.com\/company\/cri-centre-de-recherche-sur-l-inflammation\/"]}]}},"_links":{"self":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/publication\/65324","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/types\/publication"}],"acf:term":[{"embeddable":true,"taxonomy":"equipes","href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/equipes\/101"}],"wp:attachment":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/media?parent=65324"}],"wp:term":[{"taxonomy":"category_publication","embeddable":true,"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/category_publication?post=65324"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}