{"id":65318,"date":"2023-03-09T12:21:41","date_gmt":"2023-03-09T11:21:41","guid":{"rendered":"https:\/\/cri1149.fr\/?post_type=publications&p=65318"},"modified":"2025-09-11T16:47:58","modified_gmt":"2025-09-11T14:47:58","slug":"impact-of-bnt162b2-mrna-anti-sars-cov-2-vaccine-on-interferon-alpha-production-by-plasmacytoid-dendritic-cells-and-autoreactive-t-cells-in-patients-with-systemic-lupus-erythematosus-the-covalus-proje","status":"publish","type":"publication","link":"https:\/\/cri1149.fr\/en\/publication\/impact-of-bnt162b2-mrna-anti-sars-cov-2-vaccine-on-interferon-alpha-production-by-plasmacytoid-dendritic-cells-and-autoreactive-t-cells-in-patients-with-systemic-lupus-erythematosus-the-covalus-proje\/","title":{"rendered":"Impact of BNT162b2 mRNA anti-SARS-CoV-2 vaccine on interferon-alpha production by plasmacytoid dendritic cells and autoreactive T cells in patients with systemic lupus erythematosus: The COVALUS project"},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false},"category_publication":[],"class_list":["post-65318","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"J Autoimmun.","date_de_publication":"20230101","numero_doi":"","equipe":[101],"auteurs-liste":[{"texte_libre":false,"auteur-lien":21407,"auteur-text":""},{"texte_libre":false,"auteur-lien":13209,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Valentine Marie Ferr\u00e9"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Pascale Nicaise-Roland"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Diane Descamps"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Nicole Delory"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Chrystelle Fran\u00e7ois"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Celine Mendes"},{"texte_libre":false,"auteur-lien":13134,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Tiphaine Goulenok"},{"texte_libre":false,"auteur-lien":13051,"auteur-text":""},{"texte_libre":false,"auteur-lien":13151,"auteur-text":""}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC9760608\/"}],"paragraphe":"[:fr]

Abstract<\/h2>\r\n
\r\n
\r\n

Objective<\/h3>\r\n

To evaluate the specific response of SLE patients to BNT162b2 vaccination and its impact on autoimmunity defined as\u00a0in vivo<\/em>\u00a0production of interferon-alpha (IFN\u03b1) by plasmacytoid dendritic cells (pDCs) and autoreactive immune responses.<\/p>\r\n<\/div>\r\n

\r\n

Methods<\/h3>\r\n

Our prospective study included SLE patients and healthy volunteers (HV) who received 2 doses of BNT162b2 vaccine 4 weeks apart. Subjects under immunosuppressive drugs or with evidence of prior COVID-19 were excluded. IgG anti-Spike SARS-CoV-2 (anti-S) antibodies, anti-S specific-B cells, anti-S specific T cells,\u00a0in vivo<\/em>\u00a0INF-\u03b1 production by pDCs, activation marker expression by pDCs and autoreactive anti-nuclear T cells were quantified before first injection, before second injection, and 3 and 6 months after first injection.<\/p>\r\n<\/div>\r\n

\r\n

Results<\/h3>\r\n

Vaccinated SLE patients produced significantly lower IgG antibodies and specific B cells against SARS\u2013CoV-2 as compared to HV. In contrast, anti-S T cell response did not significantly differ between SLE patients and HV. Following vaccination, the surface expression of HLA-DR and CD86 and the\u00a0in vivo<\/em>\u00a0production of IFN\u03b1 by pDCs significantly increased in SLE patients. The boosted expression of HLA-DR on pDCs induced by BNT162b2 vaccine correlated with the overall immune responses against SARS-CoV-2 (anti-S antibodies: r\u00a0=\u00a00.27 [0.05\u20130.46], p\u00a0=\u00a00.02; anti-S B cells: r\u00a0=\u00a00.19 [-0.03-0.39], p\u00a0=\u00a00.09); anti-S T cells: r\u00a0=\u00a00.28 [0.05\u20130.47], p\u00a0=\u00a00.016). Eventually, anti-SARS-CoV-2 vaccination was associated with an overall decrease of autoreactive T cells (slope\u00a0=\u00a0- 0.00067, p\u00a0=\u00a00.015).<\/p>\r\n<\/div>\r\n

\r\n

Conclusion<\/h3>\r\n

BNT162b2 vaccine induces a transient\u00a0in vivo<\/em>\u00a0activation of pDCs in SLE that contributes to the immune responses against SARS-CoV-2. Unexpectedly BNT162b2 vaccine also dampens the pool of circulating autoreactive T cells, suggesting that vaccination may have a beneficial impact on SLE disease.<\/p>\r\n<\/div>\r\n<\/div>[:]","paragraphe_en":"[:fr]

Abstract<\/h2>\r\n
\r\n
\r\n

Objective<\/h3>\r\n

To evaluate the specific response of SLE patients to BNT162b2 vaccination and its impact on autoimmunity defined as\u00a0in vivo<\/em>\u00a0production of interferon-alpha (IFN\u03b1) by plasmacytoid dendritic cells (pDCs) and autoreactive immune responses.<\/p>\r\n<\/div>\r\n

\r\n

Methods<\/h3>\r\n

Our prospective study included SLE patients and healthy volunteers (HV) who received 2 doses of BNT162b2 vaccine 4 weeks apart. Subjects under immunosuppressive drugs or with evidence of prior COVID-19 were excluded. IgG anti-Spike SARS-CoV-2 (anti-S) antibodies, anti-S specific-B cells, anti-S specific T cells,\u00a0in vivo<\/em>\u00a0INF-\u03b1 production by pDCs, activation marker expression by pDCs and autoreactive anti-nuclear T cells were quantified before first injection, before second injection, and 3 and 6 months after first injection.<\/p>\r\n<\/div>\r\n

\r\n

Results<\/h3>\r\n

Vaccinated SLE patients produced significantly lower IgG antibodies and specific B cells against SARS\u2013CoV-2 as compared to HV. In contrast, anti-S T cell response did not significantly differ between SLE patients and HV. Following vaccination, the surface expression of HLA-DR and CD86 and the\u00a0in vivo<\/em>\u00a0production of IFN\u03b1 by pDCs significantly increased in SLE patients. The boosted expression of HLA-DR on pDCs induced by BNT162b2 vaccine correlated with the overall immune responses against SARS-CoV-2 (anti-S antibodies: r\u00a0=\u00a00.27 [0.05\u20130.46], p\u00a0=\u00a00.02; anti-S B cells: r\u00a0=\u00a00.19 [-0.03-0.39], p\u00a0=\u00a00.09); anti-S T cells: r\u00a0=\u00a00.28 [0.05\u20130.47], p\u00a0=\u00a00.016). Eventually, anti-SARS-CoV-2 vaccination was associated with an overall decrease of autoreactive T cells (slope\u00a0=\u00a0- 0.00067, p\u00a0=\u00a00.015).<\/p>\r\n<\/div>\r\n

\r\n

Conclusion<\/h3>\r\n

BNT162b2 vaccine induces a transient\u00a0in vivo<\/em>\u00a0activation of pDCs in SLE that contributes to the immune responses against SARS-CoV-2. Unexpectedly BNT162b2 vaccine also dampens the pool of circulating autoreactive T cells, suggesting that vaccination may have a beneficial impact on SLE disease.<\/p>\r\n<\/div>\r\n<\/div>[:]","documents":null},"yoast_head":"\nImpact of BNT162b2 mRNA anti-SARS-CoV-2 vaccine on interferon-alpha production by plasmacytoid dendritic cells and autoreactive T cells in patients with systemic lupus erythematosus: The COVALUS project - CRI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cri1149.fr\/publication\/impact-of-bnt162b2-mrna-anti-sars-cov-2-vaccine-on-interferon-alpha-production-by-plasmacytoid-dendritic-cells-and-autoreactive-t-cells-in-patients-with-systemic-lupus-erythematosus-the-covalus-proje\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Impact of BNT162b2 mRNA anti-SARS-CoV-2 vaccine on interferon-alpha production by plasmacytoid dendritic cells and autoreactive T cells in patients with systemic lupus erythematosus: The COVALUS project - 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