{"id":64054,"date":"2023-01-19T15:52:00","date_gmt":"2023-01-19T14:52:00","guid":{"rendered":"https:\/\/cri1149.fr\/?post_type=publications&#038;p=64054"},"modified":"2025-09-11T16:47:58","modified_gmt":"2025-09-11T14:47:58","slug":"card9-in-neutrophils-protects-from-colitis-and-controls-mitochondrial-metabolism-and-cell-survival","status":"publish","type":"publication","link":"https:\/\/cri1149.fr\/en\/publication\/card9-in-neutrophils-protects-from-colitis-and-controls-mitochondrial-metabolism-and-cell-survival\/","title":{"rendered":"CARD9 in neutrophils protects from colitis and controls mitochondrial metabolism and cell survival"},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false},"category_publication":[],"class_list":["post-64054","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"Gut.","date_de_publication":"20230601","numero_doi":"","equipe":[133],"auteurs-liste":[{"texte_libre":true,"auteur-lien":null,"auteur-text":"Camille Danne"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Chlo\u00e9 Michaudel"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Jurate Skerniskyte"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Julien Planchais"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Aur\u00e9lie Magniez"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Allison Agus"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Marie-Laure Michel"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Bruno Lamas"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Gregory Da Costa"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Madeleine Spatz"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Cyriane Oeuvray"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Chlo\u00e9 Galbert"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Maxime Poirier"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Yazhou Wang"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Alexia Lapi\u00e8re"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Nathalie Rolhion"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Tatiana Ledent"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"C\u00e9dric Pionneau"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Solenne Chardonnet"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Floriant Bellvert"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Edern Cahoreau"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Amandine Rocher"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Rafael Rose Arguello"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Carole Peyssonnaux"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Sabine Louis"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Mathias L Richard"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Philippe Langella"},{"texte_libre":false,"auteur-lien":13071,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Benoit Marteyn"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Harry Sokol"}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/pubmed.ncbi.nlm.nih.gov\/36167663\/"},{"lien":"https:\/\/gut.bmj.com\/content\/early\/2022\/09\/27\/gutjnl-2022-326917.long"}],"paragraphe":"<p>[:en]<\/p>\r\n<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Objectives:\u00a0<\/strong>Inflammatory bowel disease (IBD) results from a combination of genetic predisposition, dysbiosis of the gut microbiota and environmental factors, leading to alterations in the gastrointestinal immune response and chronic inflammation. Caspase recruitment domain 9 (<i>Card9<\/i>), one of the IBD susceptibility genes, has been shown to protect against intestinal inflammation and fungal infection. However, the cell types and mechanisms involved in the CARD9 protective role against inflammation remain unknown.<\/p>\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Design:\u00a0<\/strong>We used dextran sulfate sodium (DSS)-induced and adoptive transfer colitis models in total and conditional CARD9 knock-out mice to uncover which cell types play a role in the CARD9 protective phenotype. The impact of\u00a0<i>Card9<\/i>\u00a0deletion on neutrophil function was assessed by an in vivo model of fungal infection and various functional assays, including endpoint dilution assay, apoptosis assay by flow cytometry, proteomics and real-time bioenergetic profile analysis (Seahorse).<\/p>\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Results:\u00a0<\/strong>Lymphocytes are not intrinsically involved in the CARD9 protective role against colitis. CARD9 expression in neutrophils, but not in epithelial or CD11c+cells, protects against DSS-induced colitis. In the absence of CARD9, mitochondrial dysfunction increases mitochondrial reactive oxygen species production leading to the premature death of neutrophilsthrough apoptosis, especially in oxidative environment. The decreased functional neutrophils in tissues might explain the impaired containment of fungi and increased susceptibility to intestinal inflammation.<\/p>\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Conclusion:\u00a0<\/strong>These results provide new insight into the role of CARD9 in neutrophil mitochondrial function and its involvement in intestinal inflammation, paving the way for new therapeutic strategies targeting neutrophils.<\/p>\r\n<\/div>\r\n<p>[:]<\/p>","paragraphe_en":"<p>[:en]<\/p>\r\n<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Objectives:\u00a0<\/strong>Inflammatory bowel disease (IBD) results from a combination of genetic predisposition, dysbiosis of the gut microbiota and environmental factors, leading to alterations in the gastrointestinal immune response and chronic inflammation. Caspase recruitment domain 9 (<i>Card9<\/i>), one of the IBD susceptibility genes, has been shown to protect against intestinal inflammation and fungal infection. However, the cell types and mechanisms involved in the CARD9 protective role against inflammation remain unknown.<\/p>\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Design:\u00a0<\/strong>We used dextran sulfate sodium (DSS)-induced and adoptive transfer colitis models in total and conditional CARD9 knock-out mice to uncover which cell types play a role in the CARD9 protective phenotype. The impact of\u00a0<i>Card9<\/i>\u00a0deletion on neutrophil function was assessed by an in vivo model of fungal infection and various functional assays, including endpoint dilution assay, apoptosis assay by flow cytometry, proteomics and real-time bioenergetic profile analysis (Seahorse).<\/p>\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Results:\u00a0<\/strong>Lymphocytes are not intrinsically involved in the CARD9 protective role against colitis. CARD9 expression in neutrophils, but not in epithelial or CD11c+cells, protects against DSS-induced colitis. In the absence of CARD9, mitochondrial dysfunction increases mitochondrial reactive oxygen species production leading to the premature death of neutrophilsthrough apoptosis, especially in oxidative environment. The decreased functional neutrophils in tissues might explain the impaired containment of fungi and increased susceptibility to intestinal inflammation.<\/p>\r\n<p style=\"text-align: justify;\"><strong class=\"sub-title\">Conclusion:\u00a0<\/strong>These results provide new insight into the role of CARD9 in neutrophil mitochondrial function and its involvement in intestinal inflammation, paving the way for new therapeutic strategies targeting neutrophils.<\/p>\r\n<\/div>\r\n<p>[:]<\/p>","documents":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>CARD9 in neutrophils protects from colitis and controls mitochondrial metabolism and cell survival - CRI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cri1149.fr\/publication\/card9-in-neutrophils-protects-from-colitis-and-controls-mitochondrial-metabolism-and-cell-survival\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"CARD9 in neutrophils protects from colitis and controls mitochondrial metabolism and cell survival - 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