{"id":14879,"date":"2019-10-22T12:45:16","date_gmt":"2019-10-22T11:45:16","guid":{"rendered":"https:\/\/cri1149.fr\/?post_type=publications&#038;p=14879"},"modified":"2025-09-11T16:48:02","modified_gmt":"2025-09-11T14:48:02","slug":"the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology","status":"publish","type":"publication","link":"https:\/\/cri1149.fr\/en\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/","title":{"rendered":"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils."},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false},"category_publication":[],"class_list":["post-14879","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"Frontiers In Immunology.","date_de_publication":"20191101","numero_doi":"","equipe":[133],"auteurs-liste":[{"texte_libre":false,"auteur-lien":13250,"auteur-text":""},{"texte_libre":false,"auteur-lien":13028,"auteur-text":""},{"texte_libre":false,"auteur-lien":13089,"auteur-text":""},{"texte_libre":false,"auteur-lien":13174,"auteur-text":""},{"texte_libre":false,"auteur-lien":13057,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"[:fr]Shiyuan Yu[:en]Yu S[:]"},{"texte_libre":false,"auteur-lien":13071,"auteur-text":""}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/www.ncbi.nlm.nih.gov\/pmc\/articles\/PMC6839773\/"}],"paragraphe":"[:fr]<h2 id=\"abstract-1title\" class=\"head no_bottom_margin ui-helper-clearfix\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div>\r\n<p class=\"p p-first-last\" style=\"text-align: justify;\">Production of superoxide anion and other reactive oxygen species (ROS) by neutrophils has a vital role in host defense against microbes. However, over-production can induce cell injury participating to inflammation. Superoxide anion is produced by the phagocyte NADPH oxidase\/NOX2, a multicomponent enzyme system consisting of six proteins: two trans-membrane proteins (gp91<sup><em>phox<\/em><\/sup>\u00a0and p22<sup><em>phox<\/em><\/sup>) and four soluble cytosolic proteins (p40<sup><em>phox<\/em><\/sup>, p67<sup><em>phox<\/em><\/sup>, p47<sup><em>phox<\/em><\/sup>, and the small G-proteins, Rac1\/2). Phosphorylation of p47<sup><em>phox<\/em><\/sup>\u00a0on several serines regulates NADPH oxidase activation. LPS released by gram negative bacteria can enhance or prime neutrophil superoxide production in combination with other agonists such as the bacterial peptide formyl-Met-Leu-Phe (fMLP). Since the pathways involved in LPS-induced priming are not completely understood, we investigated the role of the prolyl\u00a0<em>cis\/trans<\/em>\u00a0isomerase Pin1 in this process. Two different Pin1 inhibitors, PiB, and Juglone are able to block LPS-induced priming of ROS production by human neutrophils in a concentration dependent manner. PiB and Juglone did not inhibit LPS-induced CD11b translocation neither CD62L shedding. LPS induced an increase of Pin1 activity in neutrophils similar to TNF\u03b1 and fMLP. Since the phosphorylation of p47<sup><em>phox<\/em><\/sup>\u00a0on Ser345 is critical for NADPH oxidase up-regulation, we investigated the effect of LPS on this process. Results show that LPS induced the phosphorylation of p47<sup><em>phox<\/em><\/sup>\u00a0mainly on serine 345 and induced the activation of p38MAPKinase and ERK1\/2. These results suggest that the prolyl\u00a0<em>cis\/trans<\/em>\u00a0isomerase Pin1 may control LPS-induced priming of superoxide production in human neutrophils. Pharmacological targeting of Pin1 could be a valuable approach in sepsis.<\/p>\r\n<\/div>[:]","paragraphe_en":"[:fr]<h2 id=\"abstract-1title\" class=\"head no_bottom_margin ui-helper-clearfix\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div>\r\n<p class=\"p p-first-last\" style=\"text-align: justify;\">Production of superoxide anion and other reactive oxygen species (ROS) by neutrophils has a vital role in host defense against microbes. However, over-production can induce cell injury participating to inflammation. Superoxide anion is produced by the phagocyte NADPH oxidase\/NOX2, a multicomponent enzyme system consisting of six proteins: two trans-membrane proteins (gp91<sup><em>phox<\/em><\/sup>\u00a0and p22<sup><em>phox<\/em><\/sup>) and four soluble cytosolic proteins (p40<sup><em>phox<\/em><\/sup>, p67<sup><em>phox<\/em><\/sup>, p47<sup><em>phox<\/em><\/sup>, and the small G-proteins, Rac1\/2). Phosphorylation of p47<sup><em>phox<\/em><\/sup>\u00a0on several serines regulates NADPH oxidase activation. LPS released by gram negative bacteria can enhance or prime neutrophil superoxide production in combination with other agonists such as the bacterial peptide formyl-Met-Leu-Phe (fMLP). Since the pathways involved in LPS-induced priming are not completely understood, we investigated the role of the prolyl\u00a0<em>cis\/trans<\/em>\u00a0isomerase Pin1 in this process. Two different Pin1 inhibitors, PiB, and Juglone are able to block LPS-induced priming of ROS production by human neutrophils in a concentration dependent manner. PiB and Juglone did not inhibit LPS-induced CD11b translocation neither CD62L shedding. LPS induced an increase of Pin1 activity in neutrophils similar to TNF\u03b1 and fMLP. Since the phosphorylation of p47<sup><em>phox<\/em><\/sup>\u00a0on Ser345 is critical for NADPH oxidase up-regulation, we investigated the effect of LPS on this process. Results show that LPS induced the phosphorylation of p47<sup><em>phox<\/em><\/sup>\u00a0mainly on serine 345 and induced the activation of p38MAPKinase and ERK1\/2. These results suggest that the prolyl\u00a0<em>cis\/trans<\/em>\u00a0isomerase Pin1 may control LPS-induced priming of superoxide production in human neutrophils. Pharmacological targeting of Pin1 could be a valuable approach in sepsis.<\/p>\r\n<\/div>[:]","documents":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.4 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils. - CRI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils. - CRI\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-11T14:48:02+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\\\/\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\\\/\",\"name\":\"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils. - CRI\",\"isPartOf\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#website\"},\"datePublished\":\"2019-10-22T11:45:16+00:00\",\"dateModified\":\"2025-09-11T14:48:02+00:00\",\"breadcrumb\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\\\/#breadcrumb\"},\"inLanguage\":\"en-US\",\"potentialAction\":[{\"@type\":\"ReadAction\",\"target\":[\"https:\\\/\\\/cri1149.fr\\\/publication\\\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\\\/\"]}]},{\"@type\":\"BreadcrumbList\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/publication\\\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\\\/#breadcrumb\",\"itemListElement\":[{\"@type\":\"ListItem\",\"position\":1,\"name\":\"Accueil\",\"item\":\"https:\\\/\\\/cri1149.fr\\\/en\\\/\"},{\"@type\":\"ListItem\",\"position\":2,\"name\":\"Publications\",\"item\":\"https:\\\/\\\/cri1149.fr\\\/publications\\\/\"},{\"@type\":\"ListItem\",\"position\":3,\"name\":\"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils.\"}]},{\"@type\":\"WebSite\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#website\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/\",\"name\":\"CRI - Centre de Recherche sur l'Inflammation\",\"description\":\"Centre de Recherche sur l&#039;Inflammation\",\"publisher\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#organization\"},\"potentialAction\":[{\"@type\":\"SearchAction\",\"target\":{\"@type\":\"EntryPoint\",\"urlTemplate\":\"https:\\\/\\\/cri1149.fr\\\/?s={search_term_string}\"},\"query-input\":{\"@type\":\"PropertyValueSpecification\",\"valueRequired\":true,\"valueName\":\"search_term_string\"}}],\"inLanguage\":\"en-US\"},{\"@type\":\"Organization\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#organization\",\"name\":\"CRI1149\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/\",\"logo\":{\"@type\":\"ImageObject\",\"inLanguage\":\"en-US\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#\\\/schema\\\/logo\\\/image\\\/\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/wp-content\\\/uploads\\\/2014\\\/11\\\/8.4.2014-logo-CRI.jpg\",\"contentUrl\":\"https:\\\/\\\/cri1149.fr\\\/wp-content\\\/uploads\\\/2014\\\/11\\\/8.4.2014-logo-CRI.jpg\",\"width\":215,\"height\":176,\"caption\":\"CRI1149\"},\"image\":{\"@id\":\"https:\\\/\\\/cri1149.fr\\\/#\\\/schema\\\/logo\\\/image\\\/\"},\"sameAs\":[\"https:\\\/\\\/www.facebook.com\\\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\",\"https:\\\/\\\/www.linkedin.com\\\/company\\\/cri-centre-de-recherche-sur-l-inflammation\\\/\"]}]}<\/script>\n<!-- \/ Yoast SEO plugin. -->","yoast_head_json":{"title":"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils. - CRI","robots":{"index":"index","follow":"follow","max-snippet":"max-snippet:-1","max-image-preview":"max-image-preview:large","max-video-preview":"max-video-preview:-1"},"canonical":"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/","og_locale":"en_US","og_type":"article","og_title":"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils. - CRI","og_url":"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/","og_site_name":"CRI","article_publisher":"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","article_modified_time":"2025-09-11T14:48:02+00:00","twitter_card":"summary_large_image","schema":{"@context":"https:\/\/schema.org","@graph":[{"@type":"WebPage","@id":"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/","url":"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/","name":"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils. - CRI","isPartOf":{"@id":"https:\/\/cri1149.fr\/#website"},"datePublished":"2019-10-22T11:45:16+00:00","dateModified":"2025-09-11T14:48:02+00:00","breadcrumb":{"@id":"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/#breadcrumb"},"inLanguage":"en-US","potentialAction":[{"@type":"ReadAction","target":["https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/"]}]},{"@type":"BreadcrumbList","@id":"https:\/\/cri1149.fr\/publication\/the-prolyl-isomerase-pin1-controls-lipopolysaccharide-induced-priming-of-nadph-oxidase-in-human-neutrophils-frontiers-in-immunology\/#breadcrumb","itemListElement":[{"@type":"ListItem","position":1,"name":"Accueil","item":"https:\/\/cri1149.fr\/en\/"},{"@type":"ListItem","position":2,"name":"Publications","item":"https:\/\/cri1149.fr\/publications\/"},{"@type":"ListItem","position":3,"name":"The prolyl isomerase Pin1 controls lipopolysaccharide-induced priming of NADPH oxidase in human neutrophils."}]},{"@type":"WebSite","@id":"https:\/\/cri1149.fr\/#website","url":"https:\/\/cri1149.fr\/","name":"CRI - Centre de Recherche sur l'Inflammation","description":"Centre de Recherche sur l&#039;Inflammation","publisher":{"@id":"https:\/\/cri1149.fr\/#organization"},"potentialAction":[{"@type":"SearchAction","target":{"@type":"EntryPoint","urlTemplate":"https:\/\/cri1149.fr\/?s={search_term_string}"},"query-input":{"@type":"PropertyValueSpecification","valueRequired":true,"valueName":"search_term_string"}}],"inLanguage":"en-US"},{"@type":"Organization","@id":"https:\/\/cri1149.fr\/#organization","name":"CRI1149","url":"https:\/\/cri1149.fr\/","logo":{"@type":"ImageObject","inLanguage":"en-US","@id":"https:\/\/cri1149.fr\/#\/schema\/logo\/image\/","url":"https:\/\/cri1149.fr\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","contentUrl":"https:\/\/cri1149.fr\/wp-content\/uploads\/2014\/11\/8.4.2014-logo-CRI.jpg","width":215,"height":176,"caption":"CRI1149"},"image":{"@id":"https:\/\/cri1149.fr\/#\/schema\/logo\/image\/"},"sameAs":["https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153","https:\/\/www.linkedin.com\/company\/cri-centre-de-recherche-sur-l-inflammation\/"]}]}},"_links":{"self":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/publication\/14879","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/publication"}],"about":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/types\/publication"}],"acf:term":[{"embeddable":true,"taxonomy":"equipes","href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/equipes\/133"}],"wp:attachment":[{"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/media?parent=14879"}],"wp:term":[{"taxonomy":"category_publication","embeddable":true,"href":"https:\/\/cri1149.fr\/en\/wp-json\/wp\/v2\/category_publication?post=14879"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}