{"id":13434,"date":"2017-03-20T13:52:25","date_gmt":"2017-03-20T12:52:25","guid":{"rendered":"http:\/\/cyber.pixilie.fr\/?post_type=publications&#038;p=13434"},"modified":"2025-09-11T16:48:05","modified_gmt":"2025-09-11T14:48:05","slug":"irap-endosomes-restrict-tlr9-activation-and-signaling","status":"publish","type":"publication","link":"https:\/\/cri1149.fr\/en\/publication\/irap-endosomes-restrict-tlr9-activation-and-signaling\/","title":{"rendered":"IRAP+ endosomes restrict TLR9 activation and signaling"},"content":{"rendered":"","protected":false},"featured_media":0,"template":"","meta":{"_acf_changed":false,"_EventAllDay":false,"_EventTimezone":"","_EventStartDate":"","_EventEndDate":"","_EventStartDateUTC":"","_EventEndDateUTC":"","_EventShowMap":false,"_EventShowMapLink":false,"_EventURL":"","_EventCost":"","_EventCostDescription":"","_EventCurrencySymbol":"","_EventCurrencyCode":"","_EventCurrencyPosition":"","_EventDateTimeSeparator":"","_EventTimeRangeSeparator":"","_EventOrganizerID":[],"_EventVenueID":[],"_OrganizerEmail":"","_OrganizerPhone":"","_OrganizerWebsite":"","_VenueAddress":"","_VenueCity":"","_VenueCountry":"","_VenueProvince":"","_VenueState":"","_VenueZip":"","_VenuePhone":"","_VenueURL":"","_VenueStateProvince":"","_VenueLat":"","_VenueLng":"","_VenueShowMap":false,"_VenueShowMapLink":false},"category_publication":[],"class_list":["post-13434","publication","type-publication","status-publish","hentry"],"acf":{"numero_de_publication":"Nat Immunol.","date_de_publication":"20170320","numero_doi":"","equipe":[155],"auteurs-liste":[{"texte_libre":true,"auteur-lien":null,"auteur-text":"Babdor J"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Descamps D"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Adiko AC"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Tohm\u00e9 M"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Maschalidi S"},{"texte_libre":false,"auteur-lien":13182,"auteur-text":""},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Vasconcellos LR"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"De Luca M"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Mauvais FX"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Garfa-Traore M"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Brinkmann MM"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Chignard M"},{"texte_libre":true,"auteur-lien":null,"auteur-text":"Manoury B"},{"texte_libre":false,"auteur-lien":13153,"auteur-text":""}],"auteurs-manuel":"","liens_externes":null,"liens":[{"lien":"https:\/\/www.ncbi.nlm.nih.gov\/pubmed\/28319098"}],"paragraphe":"[:fr]<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\">The retention of intracellular Toll-like receptors (TLRs) in the endoplasmic reticulum prevents their activation under basal conditions. TLR9 is activated by sensing ligands in specific endosomal-lysosomal compartments. Here we identified IRAP<sup>+<\/sup>\u00a0endosomes as major cellular compartments for the early steps of TLR9 activation in dendritic cells (DCs). Both TLR9 and its ligand, the dinucleotide CpG, were present as cargo in IRAP<sup>+<\/sup>\u00a0endosomes. In the absence of the aminopeptidase IRAP, the trafficking of CpG and TLR9 to lysosomes and signaling via TLR9 were enhanced in DCs and in mice following bacterial infection. IRAP stabilized CpG-containing endosomes by interacting with the actin-nucleation factor FHOD4, which slowed the trafficking of TLR9 toward lysosomes. Thus, endosomal retention of TLR9 via the interaction of IRAP with the actin cytoskeleton is a mechanism that prevents hyper-activation of TLR9 in DCs.<\/p>\r\n<\/div>[:]","paragraphe_en":"[:fr]<h2 class=\"title\" style=\"text-align: justify;\">Abstract<\/h2>\r\n<div id=\"eng-abstract\" class=\"abstract-content selected\">\r\n<p style=\"text-align: justify;\">The retention of intracellular Toll-like receptors (TLRs) in the endoplasmic reticulum prevents their activation under basal conditions. TLR9 is activated by sensing ligands in specific endosomal-lysosomal compartments. Here we identified IRAP<sup>+<\/sup>\u00a0endosomes as major cellular compartments for the early steps of TLR9 activation in dendritic cells (DCs). Both TLR9 and its ligand, the dinucleotide CpG, were present as cargo in IRAP<sup>+<\/sup>\u00a0endosomes. In the absence of the aminopeptidase IRAP, the trafficking of CpG and TLR9 to lysosomes and signaling via TLR9 were enhanced in DCs and in mice following bacterial infection. IRAP stabilized CpG-containing endosomes by interacting with the actin-nucleation factor FHOD4, which slowed the trafficking of TLR9 toward lysosomes. Thus, endosomal retention of TLR9 via the interaction of IRAP with the actin cytoskeleton is a mechanism that prevents hyper-activation of TLR9 in DCs.<\/p>\r\n<\/div>[:]","documents":null},"yoast_head":"<!-- This site is optimized with the Yoast SEO plugin v27.2 - https:\/\/yoast.com\/product\/yoast-seo-wordpress\/ -->\n<title>IRAP+ endosomes restrict TLR9 activation and signaling - CRI<\/title>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cri1149.fr\/publication\/irap-endosomes-restrict-tlr9-activation-and-signaling\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"IRAP+ endosomes restrict TLR9 activation and signaling - CRI\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.fr\/publication\/irap-endosomes-restrict-tlr9-activation-and-signaling\/\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta property=\"article:modified_time\" content=\"2025-09-11T14:48:05+00:00\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\/\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\/\/cri1149.fr\/publication\/irap-endosomes-restrict-tlr9-activation-and-signaling\/\",\"url\":\"https:\/\/cri1149.fr\/publication\/irap-endosomes-restrict-tlr9-activation-and-signaling\/\",\"name\":\"IRAP+ endosomes restrict TLR9 activation and signaling - 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