{"id":77638,"date":"2019-02-04T13:18:20","date_gmt":"2019-02-04T13:18:20","guid":{"rendered":"https:\/\/cri1149.fr\/flow-cytometry\/"},"modified":"2026-04-02T17:59:32","modified_gmt":"2026-04-02T17:59:32","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/cri1149.fr\/en\/platforms\/flow-cytometry\/","title":{"rendered":"Flow cytometry"},"content":{"rendered":"\n

The CRI Flow Cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers training in sample acquisition, processing and analysis techniques. <\/p>\n\n

It provides self-service access to four analyzers and a sorter, as well as occasional assistance with projects requiring mastery of this technology.<\/p>\n\n

Image cytometry is only available as a service (data acquisition by an engineer).<\/p>\n\n

The Charter must be completed, signed and submitted to the platform managers for validation before any use.<\/p>\n\n

Services on offer <\/b><\/h4>\n\n

– Analysis<\/strong><\/p>\n\n

    \n
  1. Multiple membrane and intracellular markings<\/li>\n\n\n\n
  2. Signaling pathways<\/li>\n\n\n\n
  3. Cell cycle<\/li>\n\n\n\n
  4. Apoptosis<\/li>\n\n\n\n
  5. Multiplex molecular analysis<\/li>\n\n\n\n
  6. Analysis of transfected cells .<\/li>\n<\/ol>\n\n

    – Image cytometry (Imagestream MKII)<\/strong><\/p>\n\n

    – Training<\/strong><\/p>\n\n

    – Help in setting up or improving an experimental protocol<\/strong><\/p>\n\n

    – Help in analyzing and interpreting results<\/strong><\/p>\n\n

    \u00a0<\/p>\n\n

    Our equipment<\/b><\/h4>\n\n

    Spectral cytometer<\/strong><\/h3>\n\n

    – Sony ID7000 <\/b>6 lasers<\/b>320 nm, 355 nm, 405 nm, 488 nm, 561 nm and 633 nm<\/em><\/p>\n\n

    <\/p>\n\n

    Flow cytometer :<\/strong><\/h3>\n\n

    – BD <\/b>Fortessa<\/b> X20: 22 parameters (5 lasers)<\/b>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm), UV (365nm)<\/em><\/p>\n\n

    –<\/strong><\/em> BD Fortessa: 18 parameters (4 lasers)<\/strong>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm)<\/em><\/p>\n\n

    – Guava<\/b> Easycyte<\/b>6 parameters (2 lasers)<\/b>Blue (488 nm), Red (633 nm)<\/em><\/p>\n\n

    – Imagestream MKII ( 5 lasers)<\/b><\/strong>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (642 nm), UV (375nm)<\/em><\/strong>X20, X40, X60<\/em><\/p>\n\n

    <\/p>\n\n

    Sorter :<\/strong><\/h3>\n\n

    – Melody ( 3 lasers)<\/strong>Blue (488 nm)<\/em>,<\/strong> Green-yellow (561 nm)<\/em>, <\/strong> Red (633 nm)Tri 2 channels, cloning module<\/em><\/p>\n\n

    \u00a0<\/p>\n\n

    Access on request<\/em><\/strong> <\/p>\n\n

    – BD Aria III sorter: 18 parameters (4 lasers)<\/b>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (633 nm) <\/em>on request<\/p>\n\n

    Coming to the platform<\/h5>\n\n

    Inflammation Research Centersite Bichat – 4th floor, rooms 456 and 414<\/p>\n\n

    \u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"

    The CRI Flow Cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers training in sample acquisition, processing and analysis techniques. It provides self-service access to four analyzers and a sorter, as well as occasional assistance with projects requiring mastery of this technology. Image cytometry is only […]<\/p>\n","protected":false},"author":9,"featured_media":78639,"parent":77462,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":""},"class_list":["post-77638","page","type-page","status-publish","has-post-thumbnail","hentry"],"acf":[],"yoast_head":"\nFlow cytometry - CRI<\/title>\n<meta name=\"description\" content=\"CRI's flow cytometry platform offers analyses, training and state-of-the-art equipment for cellular studies\" \/>\n<meta name=\"robots\" content=\"index, follow, max-snippet:-1, max-image-preview:large, max-video-preview:-1\" \/>\n<link rel=\"canonical\" href=\"https:\/\/cri1149.fr\/en\/platforms\/flow-cytometry\/\" \/>\n<meta property=\"og:locale\" content=\"en_US\" \/>\n<meta property=\"og:type\" content=\"article\" \/>\n<meta property=\"og:title\" content=\"Flow cytometry - CRI\" \/>\n<meta property=\"og:description\" content=\"CRI's flow cytometry platform offers analyses, training and state-of-the-art equipment for cellular studies\" \/>\n<meta property=\"og:url\" content=\"https:\/\/cri1149.fr\/en\/platforms\/flow-cytometry\/\" \/>\n<meta property=\"og:site_name\" content=\"CRI\" \/>\n<meta property=\"article:publisher\" content=\"https:\/\/www.facebook.com\/CRI-Centre-de-recherche-sur-linflammation-100116118884153\" \/>\n<meta property=\"article:modified_time\" content=\"2026-04-02T17:59:32+00:00\" \/>\n<meta property=\"og:image\" content=\"https:\/\/cri1149.fr\/wp-content\/uploads\/2021\/06\/dotplot-cyto.png\" \/>\n\t<meta property=\"og:image:width\" content=\"237\" \/>\n\t<meta property=\"og:image:height\" content=\"237\" \/>\n\t<meta property=\"og:image:type\" content=\"image\/png\" \/>\n<meta name=\"twitter:card\" content=\"summary_large_image\" \/>\n<meta name=\"twitter:label1\" content=\"Est. reading time\" \/>\n\t<meta name=\"twitter:data1\" content=\"3 minutes\" \/>\n<script type=\"application\/ld+json\" class=\"yoast-schema-graph\">{\"@context\":\"https:\\\/\\\/schema.org\",\"@graph\":[{\"@type\":\"WebPage\",\"@id\":\"https:\\\/\\\/cri1149.fr\\\/en\\\/platforms\\\/flow-cytometry\\\/\",\"url\":\"https:\\\/\\\/cri1149.fr\\\/en\\\/platforms\\\/flow-cytometry\\\/\",\"name\":\"Flow cytometry - 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