{"id":77638,"date":"2019-02-04T13:18:20","date_gmt":"2019-02-04T13:18:20","guid":{"rendered":"https:\/\/cri1149.fr\/flow-cytometry\/"},"modified":"2026-04-02T17:59:32","modified_gmt":"2026-04-02T17:59:32","slug":"flow-cytometry","status":"publish","type":"page","link":"https:\/\/cri1149.fr\/en\/platforms\/flow-cytometry\/","title":{"rendered":"Flow cytometry"},"content":{"rendered":"\n<p>The CRI Flow Cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers training in sample acquisition, processing and analysis techniques. <\/p>\n\n<p>It provides self-service access to four analyzers and a sorter, as well as occasional assistance with projects requiring mastery of this technology.<\/p>\n\n<p>Image cytometry is only available as a service (data acquisition by an engineer).<\/p>\n\n<p>The Charter must be completed, signed and submitted to the platform managers for validation before any use.<\/p>\n\n<h4 class=\"wp-block-heading\"><b>Services on offer  <\/b><\/h4>\n\n<p>&#8211; <strong>Analysis<\/strong><\/p>\n\n<ol class=\"wp-block-list\">\n<li>Multiple membrane and intracellular markings<\/li>\n\n\n\n<li>Signaling pathways<\/li>\n\n\n\n<li>Cell cycle<\/li>\n\n\n\n<li>Apoptosis<\/li>\n\n\n\n<li>Multiplex molecular analysis<\/li>\n\n\n\n<li>Analysis of transfected cells .<\/li>\n<\/ol>\n\n<p>&#8211; <strong>Image cytometry (Imagestream MKII)<\/strong><\/p>\n\n<p>&#8211; <strong>Training<\/strong><\/p>\n\n<p>&#8211; <strong>Help in setting up or improving an experimental protocol<\/strong><\/p>\n\n<p>&#8211;<strong> Help in analyzing and interpreting results<\/strong><\/p>\n\n<p>\u00a0<\/p>\n\n<h4 class=\"wp-block-heading\"><b>Our equipment<\/b><\/h4>\n\n<h3 class=\"wp-block-heading\"><strong>Spectral cytometer<\/strong><\/h3>\n\n<p><b>&#8211; Sony ID7000 <\/b><b>6 lasers<br\/><\/b><em>320 nm, 355 nm, 405 nm, 488 nm, 561 nm and 633 nm<\/em><\/p>\n\n<p><\/p>\n\n<h3 class=\"wp-block-heading\"><strong>Flow cytometer :<\/strong><\/h3>\n\n<p><b>&#8211; BD <\/b><b>Fortessa<\/b><b> X20: 22 parameters (5 lasers)<br\/><\/b><em>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm), UV (365nm)<\/em><\/p>\n\n<p><em><strong>&#8211;<\/strong><\/em><strong> BD Fortessa: 18 parameters (4 lasers)<\/strong><em><br\/>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (638 nm)<\/em><\/p>\n\n<p><b>&#8211; Guava<\/b> <b>Easycyte<\/b><b>6 parameters (2 lasers)<br\/><\/b><em>Blue (488 nm), Red (633 nm)<\/em><\/p>\n\n<p><strong>&#8211; Imagestream MKII (<b> 5 lasers)<br\/><\/b><\/strong><em>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (642 nm), UV (375nm)<\/em><strong><br\/><\/strong><em>X20, X40, X60<\/em><\/p>\n\n<p><\/p>\n\n<h3 class=\"wp-block-heading\"><strong>Sorter :<\/strong><\/h3>\n\n<p><strong>&#8211; Melody ( 3 lasers)<br\/><\/strong><em>Blue (488 nm)<\/em><strong>,<\/strong> <em>Green-yellow (561 nm)<\/em><strong>, <\/strong><em> Red (633 nm)<br\/>Tri 2 channels, cloning module<\/em><\/p>\n\n<p>\u00a0<\/p>\n\n<p><strong><em>Access on request<\/em><\/strong> <\/p>\n\n<p>&#8211; BD Aria III sorter: 18 parameters (4 lasers)<b><br\/><\/b><em>Violet (405 nm), Blue (488 nm), Green-yellow (561 nm), Red (633 nm) <\/em>on request<\/p>\n\n<h5 class=\"wp-block-heading\">Coming to the platform<\/h5>\n\n<p>Inflammation Research Center<br\/>site Bichat &#8211; 4th floor, rooms 456 and 414<\/p>\n\n<p>\u00a0<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The CRI Flow Cytometry platform is open to anyone wishing to use the equipment offered by the infrastructure. The platform offers training in sample acquisition, processing and analysis techniques. 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