Gene expression signature as a surrogate marker of microvascular invasion on routine hepatocellular carcinoma biopsies

Beaufrère A, Caruso S, Calderaro J, Poté N, Bijot JC, Couchy G, Cauchy F, Vilgrain V, Zucman-Rossi J, Paradis V.

J Hepatol. 2021 Oct 5:S0168-8278(21)02099-7. doi: 10.1016/j.jhep.2021.09.034.


Short abstract:

Microvascular invasion (MVI), a major risk factor of tumor recurrence after surgery in hepatocellular carcinoma (HCC), is only detectable by microscopic examination of the surgical specimen.  In this study, we identified, by using NanoString technology, a 6-gene signature (ROS1, UGT2B7, FAS, ANGPTL7, GMNN, MKI67) strongly associated with MVI (training set of 109 FFPE surgical HCC samples: AUC 0.82, validation set of 69 FFPE HCC biopsies:  AUC 0.74). Moreover, in multivariate analysis, the MVI signature was associated with overall survival in both sets (HR: 2.29, p=0.041). This MVI signature may be thus applied in clinical practice with routine tumor biopsy and integrated into the therapeutic strategy.