VESICULES EXTRACELLULAIRES
      

Coordinatrice du plateau
       Marion TANGUY
       CRI – 3éme étage


VIDEO MICROSCOPIE
    

Coordinateur du plateau
     Pascal NICOLE
     CRI –  3ème étage – coté A – pièce 344


TECHNICAL PLATFORM OF USSING CHAMBERS

The Ussing chambers, coupled to a voltage clamp allow to measure ex-vivo the electrical characteristics of an epithelium and thus to measure the activity of the electrogenic transporters or parameters reflecting the permeability.

The technical platform offers technical training for mounting the tissues in Chambers, and help for defining experimental design to answer specific questions and analyzing the results with appropriate softwares.

Analyzes:

  1. Measurement of electrogenic transporter activity
  2. Measurement of parameters reflecting permeability (barrier function of an epithelium)
    • Measurement of transepithelial conductance (or resistance)
    • Transfer of tracer molecules (FITC dextran-4Kd, FSA, HRP…)
  3. Measurement of molecule transfer
    • Xenobiotics
    • Drugs

Murine or human tissues studied:

  • Intestinal, pulmonary, renal epithélium…
  • Human biopsies
  • Monolayer cells cultured in transwell (Caco-2, HT-29, MDCK…)

Equipement:

  • 3 Voltage clamp (DVC 1000 – World Precision Instruments)
  • LabChart 8 acquisition and analysis software (ADInstruments)
  • 6 Ussing chambers (Physiologic Instruments)

Scientific manager:
Maude Le Gall
CRI – 2nd floor – side A – room 244

maude.le-gall@inserm.fr

Technical manager:
Alexandra Willemetz
CRI – 2nd floor – side A – room 244

alexandra.willemetz@inserm.fr